Key features and details | |
Cat. No. | MABL-204 |
Name | Anti-Arl13b mAbs |
Clone No. | AFD-N295B/66R |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, WB, IF, IHC |
Species Reactivity | Rat, Mouse |
Basic Information | |
Specificity | This antibody was specific for Arl13b. It does not cross-react with Arl13a. Arl13b is a cilium- specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B. |
Alternative Name | ADP-ribosylation factor-like protein 13B; ADP-ribosylation factor-like protein 2-like 1; ARL2-like protein 1; N295B/66 |
UniProt | Q3SXY8 |
Immunogen | This antibody was raised against a fusion protein of amino acids 208-427 of mouse Arl13b. |
Application Notes | Rat E11.5 embryonic spinal cords were used for immunofluorescence with this antibody (Ferent et al, 2019; PMID:31825820). Immunoblotting was preformed on lysated mouse tissue using this antibody (Gigante et al, 2020; PMID:32129762). Arl13bhnn MEFs were immunostained with this antibody (Revenkova et al, 2018; PMID:29592971). Immunofluorescence was preformed on mouse kidney cells using this antibody (Hwang et al, 2019; PMID:30799239). Immunohistochemistry was preformed against mouse embryos using this antibody (Fiore et al, 2020; PMID:32169553). X. tropicalis tissues were immunostained with this antibody (Saurabh et al, 2018; PMID:30377171). Immunocytochemistry was preformed on MEFs with this antibody (Muñoz-Estrada et al, 2019; PMID:31391239). This antibody was used for immunohistochemistry on mouse tissues (Seungshin et al, 2016; PMID:27261005). |
Antibody First Published | Seungshin et al. Mutations in Dnaaf1 and Lrrc48 Cause Hydrocephalus, Laterality Defects, and Sinusitis in Mice G3 (Bethesda). 2016 Aug 9;6(8):2479-87. PMID:27261005 |
Note on publication | Decribes the use of this antibody in an immunohistochemistry experiment. |
COA Information (For reference only, actual COA shall prevail) | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |