Key features and details | |
Cat. No. | MABL-202 |
Name | Anti-Shank3 mAbs |
Clone No. | AFD-N69/46R |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for the shank3. It shows no cross-reactivity against Shank1 or Shank2. Shank3 is a major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapsee formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. |
Alternative Name | SH3 and multiple ankyrin repeat domains protein 3; Proline-rich synapsee-associated protein 2; ProSAP2; SPANK-2; N69/46 |
UniProt | Q9JLU4 |
Immunogen | This antibody was raised against synthetic peptide of amino acids 840-857 of rat Shank3. |
Application Notes | This antibody was used for immunoblotting on hemibrains of wild-type, heterozygous and homozygous Shank3 mice (Bozdagi et al, 2010; PMID:21167025). hemibrains of wild-type, heterozygous and homozygous Shank3 rats were used for immunoblotting with this antibody (Harony- Nicolas et al, 2017; PMID:28139198). Half brains of mice were immunoblotted with this antibody (Kolevzon et al, 2010; PMID:21062623). Western blotting was preformed with this antibody on mouse tissue (Nikoletopoulou et al, 2017; PMID:28683289). |
Antibody First Published | Kolevzon et al. Analysis of a purported SHANK3 mutation in a boy with autism: clinical impact of rare variant research in neurodevelopmental disabilities Brain Res. 2011 Mar 22;1380:98-105. PMID:21062623 |
Note on publication | Our molecular analysis of a child with a purported disruptive mutation in SHANK3 identified by a commercial genetic testing laboratory and we provide evidence that this was not an etiological variant was presented. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
