Key features and details | |
Cat. No. | MABL-201 |
Name | Anti-GIT2 mAbs |
Clone No. | AFD-N83/48R |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, IHC |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for GIT-2. GIT-2 is a GTPase-activating protein for the ADP ribosylation factor family. |
Alternative Name | ARF GTPase-activating protein GIT2; ARF GAP GIT2; Cool-interacting tyrosine-phosphorylated protein 2; CAT-2; CAT2; G protein-coupled receptor kinase-interactor 2; GRK- interacting protein 2; N83/48 |
UniProt | Q14161 |
Immunogen | This antibody was raised against a fusion protein of amino acids 450-759 of human GIT2. |
Application Notes | This antibody is recommended for detection and analysis of GIT2 by immunohistochemistry, immunoprecipitation and western blot. For instance, the mouse version of this antibody was used to detect GIT2 by western blot in : extracts of rat brain membranes (RBM) and COS cells transiently transfected with Flag-tagged GIT1 and GIT2 and untagged Kv2.1 plasmids. Furthermore it was used to detect GIT2 by immunohistochemistry in adult rat hippocampus. |
Antibody First Published | Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360 |
Note on publication | dscribes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
