Key features and details | |
Cat. No. | MABL-150 |
Name | Anti-14G2a mAbs |
Clone No. | AFD-1A7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | activation, FACS, neutralizing, Therapeutics development, FC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for an idiotype of the anti-ganglioside GD2 antibody 14G2a and behaves as an internal image of GD2. |
Alternative Name | antibody 14G2a; idiotype of 14G2a; anti-ID; GD2; ganglioside GD2; disialoganglioside GD2 |
UniProt | |
Immunogen | The anti-idiotypic antibody 1A7 was raised by immunizing BALB/c mice with a mouse anti- GD2 antibody 14G2a. |
Application Notes | Various cancers, such as melanoma, neuroblastoma, glioma, soft tissue sarcoma, and small cell carcinoma, involve overexpression of ganglioside GD2. Traditional therapeutic approaches comprised attempts to administer anti-GD2 antibodies to deplete the cancer cells. However, such interventions often lead to the development of the immune response against those therapeutic antibodies or the cytotoxic activation against the tumor cells caused by the therapeutic was insufficient. The solution to this problem might be the generation of an anti-idiotypic antibody (Ab2) against a primary antibody that can bind GD2 (Ab1). Such an anti-idiotypic antibody (Ab2) works as an internal image of the antigen (GD2) and, upon administration to a subject with a Gd2-positive tumor, can lead to the generation of anti-anti- idiotypic antibodies (Ab3), which will be directed against GD2 and will lead to the depletion of the tumor. |
Antibody First Published | Sen et al. Preclinical evaluation in nonhuman primates of murine monoclonal anti-idiotype antibody that mimics the disialoganglioside GD2 Clin Cancer Res. 1997 Nov;3(11):1969-76. PMID:9815586 |
Note on publication | Describes the generation, characterization and preclinical trials of the anti-idiotypic antibody 1A7. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
