Anti-PrP mAbs

Cat. No.

MABL-149

Name

Anti-PrP mAbs

Clone No.

AFD-P-clone

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA

Species Reactivity

Bovine, Deer, Sheep, Syrian Hamster, Human,   Mouse

Basic Information

Specificity

This antibody binds amino acid residues   90-120 [ amino acids HG(S,N)QWNKPSKPKTN] or epitope I of the bovine prion   protein. This epitope is also present in human PrP as well as in all ungulate   PrP sequences, including cattle, deer, elk, kudu, goat, and sheep.This   antibody doesnt bind the native disease- associated PrP conformer. It can   only recognise the denatured or pretreated PrP, prepared by denaturation   and/or proteolysis for peptide-like presentation.

Alternative Name

CD230; PRNP; Major prion protein; Major   scrapie-associated fibril protein 1

UniProt

P10279

Immunogen

The original antibody was generated by   immunizing mice with amino acid sequence 96-105 of bovine prion protein.

Application Notes

Bovine spongiform encephalopathy   (BSE) is a fatal neurodegenerative prion disease affecting cattle that is transmissible   to humans, manifesting as a variant of Creutzfeldt-Jakob disease (vCJD)   likely following the consumption of meat contaminated with BSE prions. This   high affinity antibody can be used for detection of infectious prion in a   sample. P clone has been used in the development of conformation dependent   immunoassay for detection of prion protein in a sample. It can recognise   prions from cow, sheep, human, deer, hamster in a western blot assay   (PMID:12389035). P clone binds peptide derived from the unstructured region   (aa90-105) of bovine PrP (BoPrP) with an affinity of 1pM (PMID: 16962610).

Antibody First Published

Safar et al. Measuring prions causing bovine spongiform encephalopathy or chronic wasting disease by immunoassays and transgenic mice. Nat Biotechnol (2002); 20(11):1147-50.PMID:12389035

Note on publication

Describes the generation of   conformation specific antibodies towards bovien prion protein to be used in a conformation dependant immunoassay.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.