Key features and details | |
Cat. No. | MABL-151 |
Name | Anti-GluN2B/NR2B glutamate receptor mAbs |
Clone No. | AFD-N59/36.1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, IP, KO, WB, IF, IHC |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for the NR2B protein, a component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage- dependent sensitivity to magnesium. |
Alternative Name | N59/36.1R; GluN2B; Glutamate receptor ionotropic, NMDA 2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B |
UniProt | Q00960 |
Immunogen | This antibody was raised by immunising BALB/c mice with a fusion of protein amino acids 20- 271 (extracellular N-terminus) of rat NR2B. |
Application Notes | The IgG2a version of this antibody has been used in western blotting on rat cortical neurons (Plowey et al, 2014; PMID: 24874075). Co-immunoprecipitation has been preformed on extrasynaptic membranes of adult rat brains with the IgG2A version of this antibody (Chiu et al, 2019; PMID:31291571). This antibody has shown use in immunocytochemistry, immunofluorescence, immunohistochemistry or for knock out. |
Antibody First Published | Plowey et al. Mutant LRRK2 Enhances Glutamatergic Synapse Activity and Evokes Excitotoxic Dendrite Degeneration Biochim Biophys Acta. 2014 Sep; 1842(9): 1596–1603.PMID:24874075 |
Note on publication | Describes research on the mutations of the LRRK2 gene. These mutations might have something to do with parkinsons disease. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
