Key features and details | |
Cat. No. | MABL-148 |
Name | Anti-Mortalin/GRP75 mAbs |
Clone No. | AFD-N52A/42.1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, IF |
Species Reactivity | Rat, worm, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for the mortalin protein. Mortalin is a chaperone protein which plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis. |
Alternative Name | N52A/42.1R; Stress-70 protein, mitochondrial; Hspa9; 75 kDa glucose- regulated protein; GRP-75; Heat shock 70 kDa protein 9; Mortalin; Peptide-binding protein 74; PBP74; p66 MOT |
UniProt | P38647 |
Immunogen | This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 551- 766 of mouse SALM2. The antibody was off traget. |
Application Notes | The IgG1 version of this antibody was used in a western blot on COS-7 cells and on COS-1 cells (Vincente et al, 2016; PMID:28322444) (Thiffault et al, 2015; PMID:26503721).Immunofluorescense experiments with the IgG1 version of this antibody as positive control were preformed on the mouse brain (Kirmiz et al, 2018; PMID:30012696). The IgG1 version of this antibody has been used for immunoprecipitation on rat brain extracts (Bishop et al, 2018; PMID:29403353). |
Antibody First Published | Gong et al Developing high-quality mouse monoclonal antibodies for neuroscience research - approaches, perspectives and opportunities N Biotechnol. 2016 Sep 25;33(5 Pt A):551-64. PMID:26644354 |
Note on publication | Describes the need for good antibodies usable is neurological research and the creation of these antibodies |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
