Key features and details | |
Cat. No. | MABL-147 |
Name | Anti-NGL-3/LRRC4B mAbs |
Clone No. | AFD-N51/6.2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, IP, WB |
Species Reactivity | Rat, Mouse |
Basic Information | |
Specificity | This antibody is specific for NGL-3/LRRC4B. The antibody shows no cross-reactivity against NGL-1 or NGL-2. NGL-3/LRRC4B is a synaptic adhesion protein. It regulates the formation of excitatory synapses. |
Alternative Name | N51/6.2R; Leucine-rich repeat-containing protein 4B; N51/6; Lrrc4b; Netrin-G3 ligand; NGL-3 |
UniProt | P0CC10 |
Immunogen | This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 598- 709 of rat NGL-3. |
Application Notes | This antibody is recommended for detection and analysis of NGL-3 by immunocytochemistry, immunopercipitation and western blot. For instance, the mouse version of this antibody was used to detect NGL-3 by western blot in extracts of RBM and COS cells transiently transfected with Myc-tagged NGL-1, NGL-2 NGL-3 or untagged Kv2.1 plasmid. |
Antibody First Published | Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360 |
Note on publication | This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
