Anti-VGlut2 mAbs

Cat. No.

MABL-128

Name

Anti-VGlut2  mAbs

Clone No.

AFD-N29/29

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ICC, WB, IF, IHC

Species Reactivity

Chicken, Rat, Torpedo californica, Human, Mouse

Basic Information

Specificity

This antibody is specific for the Vglut1   protein. The antibody does not cross-react with VGlut1 or VGlut3. Vglut2   mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve   terminals of excitatory neural cells.

Alternative Name

N29/29R; Vesicular glutamate transporter 2; Slc17a6; Differentiation- associated BNPI; Differentiation-associated   Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17   member 6

UniProt

Q9JI12

Immunogen

This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 501- 582 (cytoplasmic   C-terminus) of rat VGlut2.

Application Notes

The IgG1 antibody was used for   immunofluorescense on the brain of a chicken (González-Cabrera et al, 2015;   PMID:25594665). Immunohistochemistry was preformed on the brain of a chicken   using the IgG1 version of this antibody (González-Cabrera et al, 2015;   PMID:25594665). The IgG1 variant of this antibody was used in a   immunocytochemistry experiment on human neurons (Fantuzzo et al, 2017;   PMID:28781993). Immunofluorescense was preformed with the IgG1 version of   this antibody on the neurons of a rat (Dabrowska et al, 2013; PMID:24009552).   The IgG1 version of this antibody was used to preform western blot and   immunofluorescense on the electroplaque of Torpedo californica (Li and   Harlow, 2014; PMID:24744885)

Antibody First Published

Andrews et al. A toolbox of IgG   subclass-switched recombinant monoclonal antibodies for enhanced multiplex   immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360

Note on publication

This article describes the generation of a library   of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing   hybridomas for neuroscience research.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.