Anti-TRIP8b (exon 1a/5) mAbs

Cat. No.

MABL-129

Name

Anti-TRIP8b (exon 1a/5)  mAbs

Clone No.

AFD-N291C/22

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ICC, WB

Species Reactivity

Rat, Mouse

Basic Information

Specificity

This antibody is specific for exon 1a/5   junction of TRIP8b. TRIP8b is an accessory subunit of   hyperpolarization-activated cyclic nucleotide-gated (HCN) channels,   regulating their cell-surface expression and cyclic nucleotide dependence.

Alternative Name

N291C/22R; PEX5-related protein; PEX5-like   protein; Peroxin-5-related protein; TPR-containing Rab8b-interacting protein;   Tetratricopeptide repeat-containing Rab8b-interacting protein; Pex5Rp

UniProt

Q925N3

Immunogen

This antibody was raised by immunising   BALB/c mice with a synthetic peptide amino acids 1- 12 (MYQGHMQLVNEQ, exon 1a   (yellow) and exon 5 (green) of constant region) of mouse TRIP8b.

Application Notes

This antibody is recommended for the   detection and analysis of TRIP8b by immunocytochemsitry and western blot. For   instance, the mouse version of this antibody was used to detect TRIP8b by   western blot in extracts of COS cells transiently transfected with   carboxyterminally (C) and amino-terminally (N) GFPtagged TRIP8b-1a-5,   TRIP8b-1a-4 or untagged Kv2.1 plasmid. Furthermore TRIP8b was also detected   by western blot in crude membranes from adult rat brain and WT and TRIP8b KO   mouse brains probed with N291C/22 and N52A/42 TC supe.

Antibody First Published

Andrews et al. A toolbox of IgG   subclass-switched recombinant monoclonal antibodies for enhanced multiplex   immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360

Note on publication

This article describes the generation of a library   of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing   hybridomas for neuroscience research.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.