Key features and details | |
Cat. No. | MABL-126 |
Name | Anti-VGlut1 mAbs |
Clone No. | AFD-N28/9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IF, IHC |
Species Reactivity | Rat |
Basic Information | |
Specificity | This antibody is specific for the Vglut1 protein. The antibody does not cross-react with VGlut2 or VGlut3. Vglut1 mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. |
Alternative Name | N28/9R; Vesicular glutamate transporter 1; Slc17a7; Brain-specific Na(+)- dependent inorganic phosphate cotransporter; Solute carrier family 17 member 7 |
UniProt | Q62634 |
Immunogen | This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 493- 560 (cytoplasmic C-terminus) of rat VGlut1 |
Application Notes | Immunofluorescense was preformed on the brain of mice and rats using this antibody (Lindhoff et al, 2009; PMID:19285470) (Garcia-Pino et al, 2009; PMID:20151361) (Takahashi et al, 2011; PMID:21262467). This antibody has been used for immunohistochemistry on the brain of a rat (Larsson et al, 2011; PMID:21810784). The antibody was used in a western blot experiment on rat brain extracts (Garcia-Pino et al, 2009; PMID:20151361). Immunocytochemistry has been preformed on normal rat retinas with this antibody (Caminos et al, 2015; PMID:25499209). |
Antibody First Published | Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360 |
Note on publication | This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
