Key features and details | |
Cat. No. | MABL-117 |
Name | Anti-Clcn4 mAbs |
Clone No. | AFD-N222/6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, WB, IHC |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for the CLCN4 protein. It does not cross react with CLCN5. CLCN4 is a strongly outwardly rectifying, electrogenic H+/Cl-exchanger which mediates the exchange of chloride ions against protons. |
Alternative Name | N222/6R; H(+)/Cl(-) exchange transporter 4; Chloride channel protein 4; ClC-4; Chloride transporter ClC-4 |
UniProt | P51793 |
Immunogen | This antibody was raised by immunising BALB/c mice with a synthetic peptide amino acids 30-51 (TDRHRKITSKSKESIWEFIKSL, cytoplasmic N-terminus) of rat Clcn4. |
Application Notes | This antibody is recommended for the detection and analysis of Clcn4 by western blot, immunocytochemistry and immunohistochemistry. For instance, the mouse version of this antibody was used to detect Clcn4 by immunohistochemistry in adult rat brain. |
Antibody First Published | Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8 |
Note on publication | This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
