Anti-TRIP8b (exon 4) mAbs

Cat. No.

MABL-116

Name

Anti-TRIP8b (exon 4)  mAbs

Clone No.

AFD-N212/3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

 IP, WB, IHC

Species Reactivity

 Rat, Human, Mouse

Basic Information

Specificity

This antibody is specific for exon 4 of   TRIP8b. It does not cross react with TRIP8b isoforms containing 1a, 1b or 2.   TRIP8b is an accessory subunit of hyperpolarization-activated cyclic   nucleotide- gated (HCN) channels, regulating their cell-surface expression   and cyclic nucleotide dependence.

Alternative Name

N212/3R; PEX5-related protein; PEX5-like   protein; Peroxin-5-related protein; TPR-containing Rab8b-interacting protein; Tetratricopeptide repeat-containing Rab8b-interacting protein; Pex5Rp

UniProt

Q925N3

Immunogen

This antibody was raised by immunising   BALB/c mice with a fusion protein amino acids 1-192 (exon 1a, exon 4 and   constant region) of rat TRIP8b.

Application Notes

Immunohistochemistry was preformed   on the brains of mice by using this antibody (Foote et al, 2019;   PMID:31492750). The antibody was used for immunoprecipitation on HEK293T   cells (Foote et al, 2019; PMID:31492750). This antibody was used for western   blotting on the proteins from rat brains (Zhou et al, 2015; PMID:25301101)

Antibody First Published

 Andrews et al. A toolbox of IgG   subclass-switched recombinant monoclonal antibodies for enhanced multiplex   immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360

Note on publication

This article describes the generation of a   library of recombinant monoclonal antibodies (R-mAbs) from a pool of   mAb-producing hybridomas for neuroscience research.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.