Anti-TRIP8b (constant) mAbs

Cat. No.

MABL-115

Name

Anti-TRIP8b (constant)  mAbs

Clone No.

AFD-N212/17

From

Recombinant Antibody

Isotype

Engineer antibody

Application

 IP, WB, IHC

Species Reactivity

 Rat, Human, Mouse

Basic Information

Specificity

This antibody is specific for the constant   region of TRIP8b, but it can cross react with several isoforms of TRIP8b.   TRIP8b is an accessory subunit of hyperpolarization-activated cyclic   nucleotide-gated (HCN) channels, regulating their cell-surface expression and   cyclic nucleotide dependence.

Alternative Name

N212/17R; PEX5-related protein; PEX5-like   protein; Peroxin-5-related protein; TPR-containing Rab8b-interacting protein; Tetratricopeptide repeat-containing Rab8b-interacting protein; Pex5Rp

UniProt

Q925N3

Immunogen

This antibody was raised by immunising   BALB/c mice with a fusion protein amino acids 1-192 (exon 1a, exon 4 and   constant region) of rat TRIP8b.

Application Notes

Immunohistochemistry was preformed   on the brains of mice by using this antibody (Foote et al+AC53, 2019;   PMID:31492750). The antibody was used for immunoprecipitation on HEK293T   cells (Foote et al, 2019; PMID:31492750). This antibody was used for western   blotting on proteins from rat brains (Zhou et al, 2015;   PMID:25301101).AD53:AF53

Antibody First Published

Andrews et al. A toolbox of IgG   subclass-switched recombinant monoclonal antibodies for enhanced multiplex   immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360

Note on publication

This article describes the generation of a   library of recombinant monoclonal antibodies (R-mAbs) from a pool of   mAb-producing hybridomas for neuroscience research.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.