Key features and details | |
Cat. No. | MABL-114 |
Name | Anti-GFAP R416WT mAbs |
Clone No. | AFD-N206B/9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, WB, IHC |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for GFAP and does not cross-react with GFAP-R416W or other proteins. GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. |
Alternative Name | N206B/9R; Glial fibrillary acidic protein; GFAP |
UniProt | P14136 |
Immunogen | This antibody was raised by immunising BALB/c mice with a synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP. |
Application Notes | This antibody is recommended for the detection and analysis of GFAP R416WT by western blot, immunocytochemistry and immunohistochemistry. For instance, the mouse version of this antibody was used to detect GFAP R416WT by western blot in brain samples from adult rat and GFAP wildtype, knockout and R416W KI mice. Futhermore it was used to detect GFAP R416WT by immunocytochemistry in GFAP WT and KO mouse hippocampus. |
Antibody First Published | Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360 |
Note on publication | This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
