Anti-Nav1.1 Na+ channel mAbs

Cat. No.

MABL-079

Name

Anti-Nav1.1 Na+ channel mAbs

Clone No.

AFD-K74/71

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IB, IP, ELISA, IF, IHC

Species Reactivity

Rat, Human, Mouse

Basic Information

Specificity

This antibody is specific for Nav1.1. It does not cross-react with Nav1.2, Nav1.3 of Nav1.6.

Alternative Name

K74/71R; Voltage-gated sodium channel subunit alpha Nav1.1; Sodium channel protein type 1 subunit alpha; Sodium channel protein brain I subunit alpha; Scn1a; NAC1; SCN1

UniProt

P04774

Immunogen

This antibody was raised by immunising BALB/c mice with a fusion protein consisting of the cytoplasmic C-terminus (amino acids 1929-2009) of   rat Nav1.1.

Application Notes

The specificity of this antibody has been confirmed in ELISA against the GST-Nav1.1 fusion protein and   immunofluorescence analysis of COS cells expressing full-length human Nav1.1   (Van Wart et al, 2005). This antibody has been used in immunoblot analysis of   adult rat brain membranes, where it labels a single band at the same position as that labelled with pan-specific anti-Nav antibody K58/35 (Van Wart et al, 2005). Additionally, this antibody has been used in immunohistochemical analysis of adult rat hippocampus and whole brain sections (Van Wart et al, 2005; Andrews et al, 2019; PMID:30667360).

Antibody First Published

Van Wart et al. Novel clustering of sodium channel Nav1.1 with ankyrin-G and neurofascin at discrete sites in the inner plexiform layer of the retina Mol Cell Neurosci. 2005 Apr;28(4):661-73. PMID:15797713

Note on publication

Describes the original generation of this antibody, and its characterisation.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.