Anti-Nerve growth factor (beta) mAbs

Cat. No.

MABL-043

Name

Anti-Nerve growth factor (beta) mAbs

Clone No.

AFD-Ig 30

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, Block, ELISA, IHC

Species Reactivity

Rat, Mouse

Basic Information

Specificity

This antibody recognises murine and rat β-NGF. It does not cross-react with BSA, aprotinin, EGF and insulin.

Alternative Name

b-NGF; β nerve growth factor; beta nerve growth factor; IgG 30; b-NGF

UniProt

Immunogen

This antibody was raised by immunising rats with polymerised β-NGF.

Application Notes

 This antibody specifically recognises native   β-NGF in ELISA experiments, and denatured β-NGF in Western blotting   (Kenigsberg et al, 1991). It has also been used to detect NGF immunoreactive sites in formaldehyde-fixed tissue (Kenigsberg et al, 1991). This antibody has been shown to block the biological activity associated with NGF, including the outgrowth of NGF-induced processes from rat pheochromocytoma cells, and the increase in choline acetyltransferase activity triggered by NGF (Kenigsberg et al, 1991).

Antibody First Published

Kenigsberg et al. Two distinct   monoclonal antibodies raised against mouse beta nerve growth factor. Generation of bi-specific anti-nerve growth factor anti-horseradish peroxidase antibodies for use in a homogeneous enzyme immunoassay. J Immunol Methods. 1991 Feb 15;136(2):247- 57. PMID:1999653

Note on publication

Describes the original generation of this antibody, and its characterisation in ELISA, WB and IHC experiments.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.