Anti-SERT mAbs

Cat. No.

MABL-044

Name

Anti-SERT mAbs

Clone No.

AFD-8B6

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallisation

Species Reactivity

Human

Basic Information

Specificity

The 8B6 Fab binds to extracellular loops 2 and 4 of hSERT, as shown by crystallography (Coleman et al, 2016).

Alternative Name

SLC6A4; Sodium-dependent serotonin transporter; 5HT transporter; Solute carrier family 6 member 4; HTT; SLC6A4

UniProt

P31645

Immunogen

8B6 was produced by immunising SERT KO mice with residues 73-616 of wildtype human SERT. Hybridomas were generated using established protocols.

Application Notes

The 8B6 Fab fragment was crystallised in complex with human SERT to aid structure determination (Coleman et al, 2016).

Antibody First Published

Coleman et al. Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram J Vis Exp. 2016; (117): 54792.   PMID:27929454

Note on publication

Describes strategies by which the human serotonin transporter was modified and crystallised. mAb 8B6 was generated using an outlined experimental rationale.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.