Anti-acetylcholine receptor mAbs

Cat. No.

MABL-042

Name

Anti-acetylcholine receptor mAbs

Clone No.

AFD-mAb198

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallisation, deplete, IP, WB, ELISA, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for the main immunogenic region of human acetylcholine receptor, which encompasses residues 67–76 of the extracellular region of the acetylcholine receptor α subunit. It cross-reacts with   acetylcholine receptors from mammalian muscle, including bovine muscle, and   from the electric organs of Torpedo rays and Electrophorus electric eels.

Alternative Name

nAchR; Neuronal acetylcholine receptor

UniProt

P02708

Immunogen

This antibody was raised by immunising rats with acetylcholine receptor derived from human skeletal   muscle.

Application Notes

The specificity of this antibody for   the major immunogenic region of human acetylcholine receptor has been confirmed in Western blot and ELISA analysis (Barchan et al, 1998). This antibody cross-links acetylcholine receptors on the muscle surface and promotes their degradation; incubation of AChR-expressing cell lines with mAb198 results in a reduction in AChR (Barchan et al, 1988). Indeed, administration of this antibody to mice is sufficient to induce experimental autoimmune   myasthenia gravis (EAMG), a neuromuscular disorder arising from a reduction   in available acetylcholine receptors at the NMJ (Asher, 1993).

Antibody First Published

Tzartos et al. Demonstration of a main immunogenic region on acetylcholine receptors from human muscle using monoclonal antibodies to human receptor. FEBS Lett. 1983 Jul 11;158(1):116-8.   PMID:6862030

Note on publication

Describes the original generation of this antibody, and characterisation of its specificity.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.