Cat. No.
MABL-3686
Application
Blocking, IHC-P, inhibition, IP, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human, Rhesus Monkey, Cynomolgus Monkey
Clone No.
MT310
From
Recombinant Antibody
Specificity
This antibody is specific for the CD4 extracellular domain D1 (binding the HIV-1 gp120-binding site).
Alternative Names
Leu3/T3; T-cell differentiation antigen; Ly-4; L3T4; T-cell surface antigen T4/Leu-3
UniProt
P01730
Immunogen
Application Notes
This antibody strongly inhibits HIV-1 gp120-binding to CD4, which blocks HIV infectivity of CD4+ T lymphocytes, as well as the binding of MHCII, which results in the block of T-cell activation (possibly leading to anergy induction) (Qin et al., 2001; PMID: n/a). Additionally, antibody binding to CD4 leads to GM-CSF, TNF-α and IL-10 induction. (TNF-α induction only occurs after 20 hours.)
Antibody First Published
Herzog et al. Monoclonal anti-CD4 in arthritis. Lancet. 1987 Dec 19;2(8573):1461-2. PMID:2892019
Note on publication
Describes the use of this antibody in immunosuppression.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
