Cat. No.
MABL-3685
Application
SPR, Block, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
chimpanzee, Human
Clone No.
CE9.1 (Clenoliximab)
From
Recombinant Antibody
Specificity
The antibody binds to human CD4 with a Kd of 1.0 nM and an ED50 of 0.2 ug/ml.
Alternative Names
p55; T cell surface antign T4/Leu-3.
UniProt
P01730
Immunogen
Domain 1 of human CD4.
Application Notes
The antibody binds specifically to CD4, a molecule which is part of the immunolgobulin super-family, expressed on the surface of helper T cells. The CD4 molecule acts as a co-receptor in the activation of T cells, aiding the interaction between the TCR and MHC II of the antigen-presenting cell. The antibody is classified as "primatized", as the variable regions of the light and heavy chains are obtained from primates, while the lamda light chain and γ4 heavy chain constant domains are human. The antibody was engineered from the primatized IgG1 monoclonal antibody Keliximab by substituting two key residues.The role of the antibody as an immunosuppressor has been tested in vivo, by intravenous administration in chimpanzees and human volunteers. The antibody reached Phase II clinical trials with Biogen-Idec.
Antibody First Published
Newman et al. Primatization of recombinant antibodies for immunotherapy of human diseases: a macaque/human chimeric antibody against human CD4. Biotechnology (NY). 1992 Nov;10(11):1455-60. PMID:1369023
Note on publication
Describes the method of producing "primatized" antibodies, where immunoglobulin genes from cynomolgus monkeys immunised with human CD4 are isolated, amplified and then cloned. The antibodies generated are against the CD4 molecule expressed on the surface of helper T cells, and are tested for both in vitro and in vivo use.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
