Cat. No.
MABL-3373
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Wheat
Clone No.
AFD-VHH26
From
Recombinant Antibody
Specificity
This antibody specifically recognizes and binds and epitope unique to wheat alpha gliadins.
Alternative Names
Alpha-gliadin; α-gliadin; Celiac Allergen; Coeliac Allergen
UniProt
Q306G0
Immunogen
The original antibody was prepared by immunization of Llama glama with whole gliadin.
Application Notes
This antibody specifically binds an epitope unique for wheat alpha gliadins which is not present in gamma or omega gliadins. It does not cross reat with rye or barley gliadins. VHH26 can detect gliadins in denaturing conditions and it retains its binding capacity in presence of 15% ethanol, 0.5% 2-mercaptoethanol, and 0.5 M guanidinium chloride. Capture ELISA using VHH26 was able to detect gliadins in samples shown as negatives by conventional ELISA. This antibody can be used for detection of gluten in commercial food samples using ELISA (limit of detection 0.24 ppm) and by competitive ELISA using conventional antibodies (limit of detection: 1 ppm) (PMID: 20039674).
Antibody First Published
Doña et al. Single domain antibodies are specially suited for quantitative determination of gliadins under denaturing conditions. J Agric Food Chem (2010); 58(2): 918-26.PMID:20039674
Note on publication
This patent describes the generation and characterisation of the 5.12.14 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
