Cat. No.
MABL-3372
Application
Blocking, inhibition, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AFD-5.12.14
From
Recombinant Antibody
Specificity
This antibody recognises Interleukin-8 (Il-8) which is a neutrophil chemotactic peptide. It is secreted by many different cells which had been activated by inflammatory stimuli. It is specific to Il-8 and does not bind to other chemokins, such as C5a, platelet factor 4 or β-TG.
Alternative Names
Interleukin-8; Il8; II-8; Il 8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factor
UniProt
P10145
Immunogen
This antibody was raised by immunising Balc/c mice against conjugated Il-8.
Application Notes
This antibody is an Il-8 antagonist and prevents binding to neutrophils, which might be used in research aiming at treating asthma (before or after its onset) (US5874080A). Furthermore, this antibody inhibits both Il-8-dependent neutrophil chemotaxis and neutrophil elastase release (US5874080A).
Antibody First Published
Hebert et al. Anti-IL-8 monoclonal antibodies for treatment of asthma Hebert, C.A., Kabakoff, R.C. and Moore, M.W., Genentech Inc, 1999. Anti-IL-8 monoclonal antibodies for treatment of asthma. U.S. Patent 5,874,080. PMID:
Note on publication
The article describes a method of generating Aspergillus-reactive IgE–secreting human hybridomas (among them these secreting this antibody). Subsequently, the antibody is characterized and its specificity is determined. Finally, this antibody is used to determin a potential presence of its antigen in the commercialy-available Aspergillus samples used in diagnosis and treatment of Aspergillus-related allergies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
