Cat. No.
MABL-3374
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
barley, rye, spelt, triticale, Wheat
Clone No.
AFD-PEE14C7
From
Recombinant Antibody
Specificity
This antibody specifically recognizes and binds deaminated gliadens and glutens. It recognizes the epitope sequence 'QPEEPFPE' and does not show any cross reactivity towards non-deamidated gliadin and gluten proteins.
Alternative Names
Deaminated gliadin; Celiac Allergen; Coeliac Allergen; Alpha-gliadin; α- gliadin; β gliadin; Beta-gliadin; γ gliadin; Gamma-gliadin; Omega-2 gliadin; Omega-5 gliadin; Omega gliadin; ω gliadin
UniProt
Immunogen
The original antibody was generated by immunizing mice with a solution of peptide 'QPEEPFPE' conjugated to KLH and of a solution of adjuvant .
Application Notes
This antibody is recommended for detection of deaminated gliadins and glutenins from wheat, rye, barley, spelt and triticale in commercial food samples (Tranquet et al., 2012). This antibody can also be used in the detection of certain allergies to deaminated glutens (PMID: 22737987).
Antibody First Published
Tranquet et al. Selection of a monoclonal antibody for detection of gliadins and glutenins: A step towards reliable gluten quantification. Journal of Cereal Science (2012); Volume 56; Issue 3; Pages 760-763. PMID:
Note on publication
Describes the generation and specific use of this antibody in the quantitive determination of gliadins.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
