Cat. No.
MABL-3371
Application
IP, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Aspergillus fumigatus (Neosartorya fumigata)
Clone No.
AFD-5C6
From
Recombinant Antibody
Specificity
This antibody recognises Asp f 9.
Alternative Names
A. fumigatus Allergen; Asp f 9; Aspf9; probable glycosidase crf1; Crh-like protein 1; glucanase; allergen: Asp f 9; allergen Asp f 9
UniProt
Q8J0P4
Immunogen
5C6 antibody was raised using a novel technique: mononuclear cells were taken from the human subjects with allergic bronchopulmonary aspergillosis and then screened for IgE secretion.Subsequently, they were used to generate hybridomas of which some were secreting anti-Asp f 9 IgE.
Application Notes
A target of the 5C6 antibody (Asp f 9) was confirmed through IP and mass spectrometry (Wurth et al., 2018). ELISA: this antibody detected Asp f 9 in a commercial Aspergillus extract ALKB in ELISA (Wurth et al., 2018). WB: 5C6 antibody was successful in detecting Asp f 9 in western blot samples from Aspergillus extracts HS and AlgryL (Wurth et al., 2018).
Antibody First Published
Wurth et al. Human IgE mAbs define variability in commercial Aspergillus extract allergen composition JCI Insight. 2018 Oct 18;3(20). pii: 123387. doi: 10.1172/jci.insight.123387. PMID:30333320
Note on publication
The article describes a method of generating Aspergillus-reactive IgE–secreting human hybridomas (among them these secreting this antibody). Subsequently, the antibody is characterized and its specificity is determined. Finally, this antibody is used to determin a potential presence of its antigen in the commercialy-available Aspergillus samples used in diagnosis and treatment of Aspergillus-related allergies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
