Cat. No.
MABL-3365
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Dog
Clone No.
AFD-D9#3116
From
Recombinant Antibody
Specificity
The antibody recognises canine IgE (likely C-epsilon 2).
Alternative Names
immunoglobulin E; canine IgE; canine immunoglobulin E; D9
UniProt
Immunogen
This antibody was raised by immunising BALB/c mice with IgE-rich pooled parasitised dog serum and subsequent creation of hybridomas.
Application Notes
ELISA: Canine IgE-specificity of the D9#3116 antibody was confirmed by ELISA tests with different types of antibodies (DeBoer et al., 1993). WB: This antibody was successfully used in western blot detection of canine IgE in experiments on the dogs' allergies against the hen egg (Shimakura et al., 2016). ELISA: fluorometric ELISA tests were used to assay canine IgE reactivity against different vaccine components in anti-vaccine allergies (Ohmori et al., 2005).
Antibody First Published
DeBoer et al. Production and characterization of mouse monoclonal antibodies directed against canine IgE and IgG. Vet Immunol Immunopathol. 1993 Aug;37(3-4):183-99. PMID:8236798
Note on publication
Describes the original generation of this antibody, and its use in ELISA analysis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
