Cat. No.
MABL-3333
Application
crystallization, ELISA
Isotype
Engineer antibody
Species Reactivity
German Cockroach (B. germanica)
Clone No.
AFD-4C3
From
Recombinant Antibody
Specificity
This antibody binds to the C-terminal lobe of Bla G 2, via an epitope containing a carbohydrate moiety (on Asn268, however non-essential for binding, though a 40-fold reduction in affinity is observed).
Alternative Names
Cockroach Allergen; Aspartic protease Bla g 2; Blattella germanica allergen
UniProt
P54958
Immunogen
Bla g 2, a major cockroach allergen.
Application Notes
This antibody binds to Bla g 2 via an epitope distinct from the epitope used by anti-Bla g 2 antibody clone 7C11 also available through Absolute Antibody. Both antibodies may be used in a sandwich ELISA.
Antibody First Published
Li et al. Carbohydrates contribute to the interactions between cockroach allergen Bla g 2 and a monoclonal antibody J Immunol. 2011 Jan 1; 186(1): 333–340. PMID:21123808
Note on publication
Describes the generation and use of this antibody in co-crystallization study.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
