Cat. No.
MABL-3334
Application
crystallization, ELISA
Isotype
Engineer antibody
Species Reactivity
Honeybee (Apis mellifera)
Clone No.
AFD-21E11
From
Recombinant Antibody
Specificity
This antibodybinds to Api m 2, at a site competing with common allergogenic IgE antibodies.
Alternative Names
Bee Venom Allergen; Hyaluronidase; Hya; Hyal; Hyaluronoglucosaminidase; Allergen Api m II
UniProt
Q08169
Immunogen
Recombinant Api m 2, a major bee venom allergen.
Application Notes
This antibody competes with naturally occurring IgE for binding to Api m2. A Fab version of this antibody was used in a co-crystallization study
Antibody First Published
Padavattan et al. Identification of a B-cell epitope of hyaluronidase, a major bee venom allergen, from its crystal structure in complex with a specific Fab. J Mol Biol. 2007 May 4;368(3):742-52 PMID:17374540
Note on publication
Describes the generation and use of this antibody in a co-crystallization study as well as in a sandwich ELISA with clone 7C11
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
