Cat. No.
MABL-3593
Application
WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
SARS-CoV-2
Clone No.
mBG86
From
Recombinant Antibody
Specificity
mBG86 is specific to SARS-CoV-2 N protein and does not react with the SARS-CoV N protein or other similar N proteins (such as MERS-CoV, HuCoV-OC43, HuCoV-NL63, HuCoV-HKU1 and HuCoV-229E N). Western blot analysis with truncated fragments of N protein suggested that its epitope resides in AA133- 179.
Alternative Names
NP; nucleoprotein; NC; Protein N; Nucleocapsid protein; SARS-CoV-2 Protein N; SARS-CoV-2 Nucleocapsid protein; SARS Coronavirus; SARS-CoV-2; SARS CoV 2; 2019-nCoV; 86
UniProt
P0DTC9
Immunogen
The original mouse IgG1 version of this antibody was raised by immunizing BALB/c mice with a recombinant SARS-Cov-2 N protein corresponding to AA133-419.
Application Notes
Specificty of the original mouse IgG1 version of this antibody to SARS-CoV-2 was confirmed by ELISA (Terry et al., 2021; pmid: 33714753). mBG86 is a particularly useful antibody as it can be used to detect SARS-Cov-2 N protein and differentiate it from SARS-CoV. Western blot analysis demonstrated that this antibody exhibits two band labeling, suggesting a specificity for conformational epitopes on the N protein (Terry et al., 2021; pmid: 33714753). This antibody was shown to detect SARS- CoV-2 N protein in methanol-stained Vero cells by immunofluorescence (Terry et al., 2021; pmid: 33714753). Finally, the original version of mBG86 was demonstrated to be an effective detection antibody in a sandwich ELISA assay suggesting it's potential use in SARS-Cov-2 diagnostics (Terry et al., 2021; pmid: 33714753).
Antibody First Published
Terry et al. Development of SARS-CoV-2 Nucleocapsid Specific Monoclonal Antibodies Terry JS, Anderson LB, Scherman MS, McAlister CE, Perera R, Schountz T, Geiss BJ. Development of a SARS-CoV-2 nucleocapsid specific monoclonal antibody. Virology. 2021 Jun;558:28-37. PMID:33714753
Note on publication
Describes the generation and characterization of novel anti-SARS-CoV-2 N protein antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
