Cat. No.
MABL-3594
Application
NTRL, SurfacePlasmonResonance, therapeutic, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
SARSCoronavirus2 (SARS-Cov-2)
Clone No.
EY6A
From
Recombinant Antibody
Specificity
This antibody binds tightly (KD of 2 nM) to the receptor binding domain (RBD) the of the SARS- CoV-2. It also cross reacts with SARS-CoV-1. This antibody recognizes a highly conserved epitope on away from the ACE2 receptor binding domain.
Alternative Names
SARS CoV 2 S glycoprotein; COVID-19 Spike protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV
UniProt
P0DTC2
Immunogen
The original antibody was isolated by cloning antibody genes from blood derived plasmablasts of a Covid-19 infected patient in the convalescent phase.
Application Notes
EY6A binds S1 domain of SARS-CoV-2 and also cross reacts with SARS-CoV-1. The binding was confirmed by ELISA. Further confirmation of binding of EY6A to SARS-CoV-2 infected cells was done by immunofluorescence. Surface plasmon resonance (SPR) measurements for EY6A Fab showed high affinity binding to immobilised SARS-CoV-2 RBD (KD = 2 nM). EY6A did not completely block binding of ACE2 to RBD, but was successful in neutralizing SARS-CoV-2 infection in Vero E6 cells (Zhou et al., 2020).
Antibody First Published
Zhou et al. Structural basis for the neutralization of SARS-CoV-2 by an antibody from a convalescent patient. BioRxiv (2020)
Note on publication
Describes the generation, characterization and structural complex formed by this antibody with the receptor binding domain of the SARS-CoV-2.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
