Cat. No.
MABL-3592
Application
WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
SARS-CoV, SARS-CoV-2
Clone No.
mBG17
From
Recombinant Antibody
Specificity
mBG17 strongly recognizes SARS-CoV-2 N protein and SARS-CoV N protein. To lesser extent, it cross-reacts with the HuNL63 N protein. Western blot analysis with truncated fragments of N protein suggested that its epitope resides in AA381-419 at the C-terminal.
Alternative Names
NP; nucleoprotein; NC; Protein N; Nucleocapsid protein; SARS-CoV-2 Protein N; SARS-CoV-2 Nucleocapsid protein; SARS Coronavirus; SARS-CoV-2; SARS CoV 2; 2019-nCoV; 17; SARS-CoV nucleocapsid
UniProt
P0DTC9
Immunogen
The original mouse IgG1 version of this antibody was raised by immunizing BALB/c mice with a recombinant SARS-Cov-2 N protein corresponding to AA133-419.
Application Notes
Specificty of the original mouse IgG1 version of this antibody to SARS-CoV-2 and SARS- Cov was confirmed by Specificty of the original mouse IgG1 version of this antibody to SARS-CoV-2 and SARS-Cov was confirmed by ELISA (Terry et al., 2021; pmid: 33714753). This antibody detected SARS-Cov-2 N protein in Western blot exhibiting labeling patterns suggesting a linear epitope (Terry et al., 2021; pmid: 33714753). mBG17 was shown to be very effective in staining SARS-Cov-2 N protein in immunofluorescence in SARS-Cov-2-infected Vero cells, both methanol and paraformaldehyde fixed (Terry et al., 2021; pmid: 33714753). Finally, the original version of mBG17 was demonstrated to be an effective detection antibody in a sandwich ELISA assay suggesting it's potential use in SARS-Cov-2 diagnostics (Terry et al., 2021; pmid: 33714753).
Antibody First Published
Terry et al. Development of SARS-CoV-2 Nucleocapsid Specific Monoclonal Antibodies Terry JS, Anderson LB, Scherman MS, McAlister CE, Perera R, Schountz T, Geiss BJ. Development of a SARS-CoV-2 nucleocapsid specific monoclonal antibody. Virology. 2021 Jun;558:28-37. PMID:33714753
Note on publication
Describes the generation and characterization of novel anti-SARS-CoV-2 N protein antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
