Cat. No.
MABL-3591
Application
neutralising, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Bovinecoronavirus
Clone No.
SF-3E4
From
Recombinant Antibody
Specificity
This antibody recognises an epitope in the S1 subunit of the BCV spike protein.
Alternative Names
BCV
UniProt
Immunogen
This antibody was isolated from an immunised bovine scFv phage display library. The antibody repertoire of a Holstein Frisian bull immunized against BCV was amplified, and the antibody- encoding cDNA from isolated peripheral blood mononuclear cells was use
Application Notes
An scFv version of this antibody exhibits good binding kinetics (Km = 595 pM) and neutralization ability (≥ 0.24 μg/ml) against BCV-V270 in ELISA analysis and neutralisation assays using BCV-V270-infected VERO cell cultures (Fischer, 2017). Western blotting and a competition ELISA with BCV- V270 have shown that this scFv recognises an epitope in the BCV spike protein (Fischer, 2017). In ELISA analysis, this scFv cross-reacts with BCV-L9 and the human coronavirus strain OC43 (Fischer, 2017). This scFv is a potential therapeutic candidate for the treatment of calf diarrhea caused by coronaviruses.
Antibody First Published
Fischer Cytokines and immunoglobulin genetics in neonatal calf diarrhea Doctoral Dissertation, Faculty of Veterinary Medicine, Justus Liebig University Giessen
Note on publication
Describes the original generation of this scFv, and its characterisation in ELISA and WB analysis
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
