Cat. No.
MABL-3581
Application
WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
SARSCoronavirus
Clone No.
A11
From
Recombinant Antibody
Specificity
This antibody specifically binds the epitope in the S2 domain of the spike protein in the region of Leu803-Ala828.
Alternative Names
Spike protein; S protein; SARS-CoV S protein; S glycoprotein; E2; Peplomer protein; Spike protein S2
UniProt
P59594
Immunogen
The original antigen was produced by subcloning the scFv gene fragment in an expression vector like pET32b, and transforming the vector into an E.coli strain BL21 and inducing expression by IPTG ( isopropyl-b-D-thiogalactopyranoside). A scFv phage antibody library was constructed in fd phage. The antibody was produced by panning against S2b, two non-immune human Ab phage display libraries and neutralizing activity was tested against pseudotyped viruses. Antigen binding antibodies were identified by phage ELISA.
Application Notes
This antibody binds the amino acids Leu803-Ala828 in the S2 domain of the SARS- CoV. S2 domain mediates membrane fusion between the virus and the host cell. The binding specificity of antibody to S2b fragment of the viruse was checked by ELISA. Immunofluorescence staining was used to stain infected Vero cells using the A11-scFv. A11-scFv recognized recombinant spike protein S2-b during western blot analysis (Leung et al., 2008).
Antibody First Published
Leung et al. Development of human single-chain antibodies against SARS- associated coronavirus. Intervirology. (2008); 51(3):173-81. PMID:18724064
Note on publication
Describes the development of a single chain antibody against the S2 domain of the spike protein of the SARS coronavirus.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
