Cat. No.
MABL-3580
Application
NTRL, ELISA
Isotype
Engineer antibody
Species Reactivity
SARSCoronavirus
Clone No.
256
From
Recombinant Antibody
Specificity
This antibody specifically binds the amino acids 472 and 480 in the S1 domain of the SARS- CoV Spike protein (Tor2 and GD03).
Alternative Names
Spike protein; S protein; SARS-CoV S protein; S glycoprotein; E2; Peplomer protein; Spike protein S1
UniProt
P59594
Immunogen
The original antibody was produced by panning against GD03-RBD-C9, two non-immune human Ab phage display libraries and neutralizing activity was tested against pseudotyped viruses.
Application Notes
This antibody inhibits the association of SARS-CoV S protein to the angiotensin- converting enzyme 2 (ACE2) receptor by binding to specific amino acids on the S1 domain of the spike protein of the virus. 256-IgG1 was capable of neutralizing both the mutations of the spike protein at D480A and D480G. It also neutralized Tor2 and marginally neutralized GD03. Neither of the antibodies 11A nor 80R competed with 256 IgG1 for binding to Tor2 suggesting that the binding epitope of 256 was different from that of 80R and 11A (Sui et. al, 2008)
Antibody First Published
Sui et al. Broadening of neutralization activity to directly block a dominant antibody-driven SARS-coronavirus evolution pathway. PLoS Pathog. (2008); 4(11):e1000197. PMID:18989460
Note on publication
Describes the methods for generation of a broadly neutralizing antibody by using a combination of chain-shuffling as well as hot-spot CDR mutagenesis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
