Cat. No.
MABL-3582
Application
NTRL, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
SARSCoronavirus
Clone No.
F26G9
From
Recombinant Antibody
Specificity
This antibody specifically binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein. The originally characterized human IgG1 had a binding affinity of KD=10.2 (±3.5) nM.
Alternative Names
Spike protein; S protein; SARS-CoV S protein; S glycoprotein; E2; Peplomer protein; Spike protein S1
UniProt
P59594
Immunogen
The original antibody was generated by immunizing the BALB/C mice with the Tor-3 strain of the SARS-CoV.
Application Notes
This antibody is a SARS-CoV neutralizing antibody. F26G9 was capable of recognising both the S protein and the RBD in an ELISA. The neutralizing mAb, F26G9 specifically recognize SARS-HCoV infected but not uninfected Vero cells in immunofluorescence Immunofluorescence (Berry et al, 2004).
Antibody First Published
Berry et al. Development and characterisation of neutralising monoclonal antibody to the SARS-coronavirus Journal of Virological Methods (2004); Issue: 1; Volume: 120; Pages: 87-96. PMID:15234813
Note on publication
Describes the development and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
