Cat. No.
MABL-3579
Application
NTRL, ELISA
Isotype
Engineer antibody
Species Reactivity
SARSCoronavirus
Clone No.
11A
From
Recombinant Antibody
Specificity
This antibody specifically binds the amino acids 472 and 480 in the S1 domain of the SARS- CoV Spike protein (GD03).
Alternative Names
Spike protein; S protein; SARS-CoV S protein; S glycoprotein; E2; Peplomer protein; Spike protein S1
UniProt
P59594
Immunogen
The original antibody was produced by panning against GD03-RBD-C9, two non-immune human Ab phage display libraries and neutralizing activity was tested against pseudotyped viruses.
Application Notes
This antibody inhibits the association of SARS-CoV S protein to the angiotensin- converting enzyme 2 (ACE2) receptor by binding to specific amino acids on the S1 domain of the spike protein of the virus strain GD03. 11A potently inhibited GD03-RBD’s binding to ACE2 expressing 293T cells. Kinetic analysis of 11A IgG binding to GD03-RBD demonstrated high affinity interaction (KD = 2.260.7 nM). Single amino acid changes at either position D480A or D480G resulted in complete loss of 11A binding. (Sui et al., 2008)
Antibody First Published
Sui et al. Broadening of neutralization activity to directly block a dominant antibody-driven SARS-coronavirus evolution pathway. PLoS Pathog. (2008); 4(11):e1000197.PMID:18989460
Note on publication
Describes the methods for generation of a broadly neutralizing antibody by using a combination of chain-shuffling as well as hot-spot CDR mutagenesis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
