Key features and details | |
Cat. No. | MABL-3433 |
Name | Anti-MBP mAbs [sAb-70](MABL-3434) |
Clone No. | sAb-70 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Escherichia coli |
Basic Information | |
Specificity | This antibody is a conformation specific antibody and binds the maltose binding protein in its open conformation. It is reported that sAB-7O binds a large endosteric site on MBP using its heavy-chain CDR-H1, -H2, and -H3 and light chain CDR-L1 and -L3 residues. |
Alternative Name | malE; MMBP; Maltose-binding periplasmic protein; Maltodextrin-binding protein |
UniProt | P0AEY0; P0AEX9 |
Immunogen | This conformationally selective synthetic antibody was generated by customized phage display selections that stabilize MBP in different conformational states. |
Application Notes | This antibody binds MBP with KD of 1 nM in the absence of maltose and 152 nM in 1 µM maltose and does not bind in 1 mM maltose. This antibody binds to the maltose binding pocket of MBP with sufficient affinity to effectively outcompete maltose binding and driving the equilibrium to the open form. Therefore, in presence of excess sAB-7O there was no appreciable observation of maltose binding. Competitive ELISA was used to determine that the open (sAB-7O) and closed conformation (sAB-11M) specific antibodies compete for binding site of MBP (PMID: 29321208). |
Antibody First Published | Mukherjee et al. Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins. J Biol Chem. (2018); 293(8); 2815-2828. PMID:29321208 |
Note on publication | Describes the conformational structure and characterization of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
