Cat. No.
MABL-3980
Application
IHC-P, in vivo, IP, WB, ELISA, IF, IHC-Fr
Isotype
Engineer antibody
Species Reactivity
Monkey, Rabbit, Rat, Human, Mouse
Clone No.
PAb421
From
Recombinant Antibody
Specificity
Recognises the mammalian mutant and wild-type p53 protein and reacts with an epitope between amino acid residues 370 and 378 (near the C-terminus of the protein), corresponding to the sequence "KKGQSTSRHK".
Alternative Names
Tumor suppressor p53; Cellular tumor antigen p53
UniProt
P04637
Immunogen
This antibody was raised by immunising mice with human milk fat globule membrane
Application Notes
The binding specificity of this antibody for HMFGM has been confirmed in ELISA analysis (Aboud-Pirak et al, 1988). Once bound to its antigen on the plasma membrane of human breast carcinoma MCF-7, this antibody is rapidly endocytosed and delivered to lysosomes for digestion, as established in radiolabelling and fractionation studies (Aboud-Pirak et al, 1988). An 111lndium-labeled human-engineered form of this antibody has been shown to be a suitable carrier for cytotoxic agents in ovarian carcinoma patients (van Hof et al, 1996). Equally, when conjugated to calicheamicin amide, this antibody can target the antitumor antibiotic into MUC1-positive solid tumors (Hamann et al, 2005).
Antibody First Published
Aboud-Pirak et al. Binding and endocytosis of a monoclonal antibody to a high molecular weight human milk fat globule membrane-associated antigen by cultured MCF-7 breast carcinoma cells. Cancer Res. 1988 Jun 1;48(11):3188-96. PMID:3365702
Note on publication
Describes the original generation of this antibody, and its preliminary characterisation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
