Cat. No.
MABL-3957
Application
inhibit, IP, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Epstein-Barr virus (EBV)
Clone No.
H3
From
Recombinant Antibody
Specificity
H3 scFv is specific to the LMP1 C terminal region where it binds to CTAR-1 (GST-LMP1[187- 242]). LMP1 is expessed constitutively in many EBV-associated tumours and this protein can cause B lymphocyte and rodent fibroblast transformation, cell apoptosis inhibition by up-regulation of anti-apoptotic proteins, cell surface marker and DNA methyltransferase activty up-regulation, and cell adhesion molecule and cyclin-dependent kinase downregulation. The C-terminal domain of LMP1 is made up of 3 C-terminal activating regions (CTARs) which interact with TNF receptor-associated proteins and TNF receptor- associated death domain protein, which medite activation of NF-kB and AP-1. A LMP1 TAR is also involved in the JAK/STAT pathway.
Alternative Names
latent membrane protein 1; LMP1; LMP-1; p63; protein p63; protein p25
UniProt
P03230
Immunogen
m5Cyd-KLH conjugate.
Application Notes
The antibody binds specifically to 5-Methylcytosine, a methylated form of the nucleobase cytosine, a component of both DNA and RNA. In DNA, the presence of 5mC is an epigenetic mark for genome stability during embryonic development. In RNA, 5-methylcytidine is associated with structural stability, aminoacylation and codon recognition of tRNA. The monoclonal antibody FMC9 can be used for detection of methylated DNA in patient blood serum, and can be detected in the urine or tissue of patients with certain cancers, including bladder, breast and colon.
Antibody First Published
Mizugaki M, Itoh K, Yamaguchi T, Ishiwata S, Hishinuma T, Nozaki S, Ishida N. Preparation of a monoclonal antibody specific for 5-methyl-2'-deoxycytidine and its application for the detection of DNA methylation levels in human peripheral blood cells. Biol Pharm Bull. 1996 Dec;19(12):1537-40. PMID:8996634
Note on publication
Describes the preparation of the monoclonal antibody FMC9, specific for the modified nucleoside 5-methyl-2'-deoxycytidine (m5dCyd). The antibody was prepared using 5- methylcytidine -keyhole limpet haemocyanin (KLH) conjugate. Also describes how the FMC9 antibody can be used in monitoring m5dCyd levels in peripheral blood cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
