Cat. No.
MABL-3958
Application
ICC, IPP, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
FMC-9
From
Recombinant Antibody
Specificity
The antibody binds to m5dCyd with an IC50 of 4.27 μg/ml.
Alternative Names
5-methylcytosine
UniProt
Immunogen
The original antibody was isolated from a patient suffering from a tumor expressing a tumor specific antigen like MAGE-A3.
Application Notes
The binding specificity of this antibody towards MAGE-A3 was determined using ELISA. The original antibody cross reacts with MAGEA6 but does not react with other members of the MAGE family in an ELISA or western blot assay. This antibody was used in the detection of HEK293T cells expressing MAGEA3 and MAGEA6 using flow cytometry. The original IgG1 antibody was capable of immunoprecipitating MAGE-A3 from lysates of SK-MEL-37 cells endogenously expressing MAGEA3. The original antibody was also used in the immunohistochemical staining of tissues form normal testis and squamous cell carcinoma of the lung (US20140093514).
Antibody First Published
Esslinger et al. Magea3 binding antibodies. (US20140093514)
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
