Cat. No.
MABL-3956
Application
ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
H18A
From
Recombinant Antibody
Specificity
Alternative Names
Lewis Y
UniProt
Immunogen
H3 was selected by its affinity for GST-LMP1 from a human phage display library after 4 rounds of panning.
Application Notes
H3 scFv can reduce LMP1-mediated NF-kappaB activation in HEK293 cells by ~50% as well as inhibit LMP1 functions in epithelial cells - enables the potential use of H3 for attenuating the LMP1 function in LMP1-positive tumors. H3 is also capable of reducing the trans-membrane migration ability of MDCK-LMP1 cells by blocking the CTAR-mediated signaling pathway. H3 can also be used to pull down LMP1 in IPs, and can be used for ELISAs and to visualise LMP1 distibution in IF studies.
Antibody First Published
Fang et al. Modulation of Epstein-Barr virus latent membrane protein 1 activity by intrabodies. Intervirology. 2007;50(4):254-63. Epub 2007 Apr 25. PMID:17460414
Note on publication
Describes the generation of and characterization of a number of scFvs specific for different regions of the LMP1 C terminal domain.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
