Cat. No.
MABL-3952
Application
WB, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
6F4
From
Recombinant Antibody
Specificity
This antibody specifically recognizes human junctional adhesion molecule A and does not cross-react with other members of the JAM family. JAM-A is an adherens and tight junction protein expressed by endothelial and epithelial cells. It is recently identified as an antigenic protein (Ag) overexpressed in tumor cells and associated with cancer progression.
Alternative Names
CD321; JAM-1; JAM1; JCAM; F11R; PAM-1; Junctional adhesion molecule A; Junctional adhesion molecule 1; Platelet F11 receptor
UniProt
Q9Y624
Immunogen
The original antibody was generated by immunizing mice with a synthetic dodecapeptide 'KLVVVGADGVGK'.
Application Notes
The binding reactivity and epitope characterization of this antibody towards human KRAS G12D mutant was determined using ELISA (PMID: 9440050). This antibody can bind both the peptide 'KLVVVGADGVGK' conjugated BSA as well as KRAS G12D mutant in an ELISA.
Antibody First Published
Wang et al. Epitopic characterization of the human wild-type and mutant ras proteins using membrane-bound peptides. J Pept Res. 1997 Dec;50(6):483-92. PMID:9440050
Note on publication
Describes the epitope characterization of many anti-ras protein antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
