Cat. No.
MABL-3951
Application
in vivo, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
4.43
From
Recombinant Antibody
Specificity
This antibody binds human IGFBP7, also known as IGFBP-related protein 1, is a 31 kDa secreted protein that share structural homology with a family of IGFBP-related proteins. It binds IGF-I and IGF-II with a relatively low affinity and stimulates prostacyclin (PGI2) production. Several studies demonstrated that IGFBP7 is overexpressed in tumor blood vessels with little or no expression in normal blood vessels nor in the non-malignant angiogenic placental tissues.
Alternative Names
IBP-7; IGFBP-7; MAC25; PSF; IGFBP-rP1; MAC25 protein; TAF; IGF-binding protein 7; Insulin-like growth factor-binding protein 7; PGI2-stimulating factor; Prostacyclin-stimulating factor; Tumor-derived adhesion factor; angiomodulin
UniProt
Q16270
Immunogen
The original antibody was generated by immunizing BALB/c mice with MCF-7 cells. The humanized version was generated by grafting CDRs of parental mouse antibody onto human framework regions.
Application Notes
This antibody was used for the characterization of human junctional adhesion molecule-A (JAM-A) and study of its expression on various cancerous tissues. The binding specificity of this antibody for human JAM-A expressed on CHO cells was confirmed using flow cytometry. JAM-A expression level on cell surface was determined by immunofluorescence staining and visualized by confocal microscopy. This antibody can also be used in the determination of JAM-A protein using western blot. Immunohistochemical analysis using this antibody revealed that JAM-A is overexpressed on breast, lung and kidney tumor tissues. Injections of anti-JAM-A antibody resulted in a significant tumor growth inhibition of xenograft human tumors in vivo. Treatment with monoclonal antibody induced a decrease of the Ki67 expression and downregulated JAM-A levels. This antigen may play a role in interfering with tumor proliferation (PMID: 22886345). This antibody binds the extracellular domain of JAM-A in an ELISA (Goetsch et al, 2011).
Antibody First Published
Goetsch et al. A novel role for junctional adhesion molecule-A in tumor proliferation: modulation by an anti-JAM-A monoclonal antibody. Int J Cancer. 2013 Mar 15;132(6):1463-74. PMID:22886345
Note on publication
Describes the characterization of a new target expressed on cancerous tissues using this antibody
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
