Cat. No.
MABL-3953
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
D113
From
Recombinant Antibody
Specificity
This antibody binds the G12D mutant form of human KRAS protein. This ras protein binds GDP/GTP and possess intrinsic GTPase activity, that results in conversion of nucleotide guanosine triphosphate (GTP) into guanosine diphosphate (GDP). This protein plays an important role in many signal transduction pathways and regulation of cell proliferation. Somatic KRAS mutations are highly observed in leukemias, colorectal cancer, pancreatic cancer and lung cancer. KRAS mutation is G12D which is estimated to be present in up to 37% pancreatic cancers and over 12% of colorectal cancers.
Alternative Names
K-Ras; GTPase Kras; KRAS2; RASK2; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; ras p21 protein; p21 ras; D113-2-1
UniProt
P01116-2
Immunogen
3S193 (IgG3) was generated in BALB/c mice by immunisation with Ley-expressing cells of the MCF-7 breast carcinoma cell-line.
Application Notes
The murine 3S193 showed high specificity for Ley in ELISA tests with synthetic Ley and Ley-containing glycoproteins and glycolipids and also reacted strongly in rosetting assays and cytotoxic tests with Ley-expressing cells (Scott et al, 2000). Murine 3S193 was humanised and in antibody-dependent cell-mediated cytotoxicity assays displayed 100-fold greater activity (Kitamura et al, 1994). 3S193 has been used to screen tumour samples for Ley expression using immunohistochemistry (Krug et al, 2007). A crystal structure of the humanised antibody has been obtained to 1.9 Å resolution (Ramsland et al, 2004).
Antibody First Published
Kitamura et al, 1994. Specificity analysis of blood group Lewis-y (Le(y)) antibodies generatedagainst synthetic and natural Le(y) determinants. Proc Natl Acad Sci U S A. 1994 Dec 20; 91(26): 12957–12961 PMID:7809154
Note on publication
Describes the original generation of the antibody and its application to ELISA.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
