Cat. No.
MABL-3950
Application
in vivo, WB, Block, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
093G9
From
Recombinant Antibody
Specificity
This antibody binds an epitope comprising amino acids 77 to 80, which lie in the C‐terminus of the IGFBP domain of CCN1 (Cyr61). A cyclic peptide encompassing residues 75 to 81 of CCN1 displays high binding affinity for 093G9 (PMID: 28608634). CYR61 is a secreted signaling molecule that promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. This molecule has been implicated as an important mediator in proliferation and metastasis of breast cancer, which indicated that blockage of Cyr61 might be a potent target for breast cancer treatment. Apart from cancer this molecule also plays a role in inflammation, liver fibrosis, and even autoimmune diseases.
Alternative Names
CCN1; CYR61; GIG1; IBP-10; IGFBP-10; CCN family member 1; Cellular communication network factor 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IGF-binding protein 10; Protein CYR61; Protein GIG1
UniProt
O00622
Immunogen
The original antibody was generated by immunizing a male llama with recombinant human IGFBP7 protein (Genbank BC017201). An immune llama library was developed and the antibody was isolated by panning against purified human IGFBP7 protein.
Application Notes
Antibody First Published
Iqbal et al. Molecular imaging of glioblastoma multiforme using anti-insulin- like growth factor-binding protein-7 single-domain antibodies. Br J Cancer. 2010 Nov 9;103(10):1606-16.PMID:20959824
Note on publication
Describes the generation of single domain antibodies against IGFBP7 and its use in molecular imaging of glioblastoma multiforme (GBM) tumor vessels.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
