Cat. No.
MABL-3916
Application
WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
OV-TL 12/30
From
Recombinant Antibody
Specificity
This antibody recognizes cytokeratin-7. Cytokeratins are a subfamily of intermediate filament proteins but this antibody does not recognize other intermediate filament proteins. The antibody reacts with proteins that are found in most ductal, glandular, transitional, and biliary duct epithelial cells.
Alternative Names
CK-7; Keratin, type II cytoskeletal 7; Cytokeratin-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7; CK7; CK 7
UniProt
P08729
Immunogen
The original antibody was generated by immunizing BALB/c mice with purified human gastrointestinal tumor derived cytokeratin.
Application Notes
This antibody has specific reactivity for human cytokeratin 8. The binding characterization and epitope mapping of this antibody was done using ELISA (PMID: 9486565; 9892182). This antibody was also capable of immunoprecipitating human cytokeratin 8 from a sample. This antibody can be used in the identification of cytokeratin 8 in a western blot assay. This antibody was also used in the immunohistochemical staining of frozen sections of normal human adult tissues like skin, esophagus and liver (PMID: 2479674). This antibody is capable of binding cytokeratin 8 expressed on carcinomas in vivo.This antibody was used for the radio immunolocalization of cytokeratin 8 and radioimmunotherapy (PMID: 9892182; 7493362; 9406704).
Antibody First Published
Sundström et al. Diversity in immunoreactivity of tumor-derived cytokeratin monoclonal antibodies. J Histochem Cytochem. 1989 Dec;37(12):1845-54. PMID:2479674
Note on publication
Describes the generation and characterization of 10 antibodies against cytokeratin which are expressed on normal and malignant epithelial cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
