Cat. No.
MABL-3915
Application
CHIP, IHC-P, IP, WB, FC, IF, IHC-Fr
Isotype
Engineer antibody
Species Reactivity
Guinea Pig, Rat, Zebrafish, Human, Cynomolgus Monkey, Mouse
Clone No.
25B6
From
Recombinant Antibody
Specificity
Epitope mapping has revealed that the 25B6 anti-CTIP2 monoclonal antibody recognizes an antigen within the 150 amino acids located at the extreme amino terminus of the protein, CTIP2-(1–150).
Alternative Names
BCL11B
UniProt
Q9C0K0
Immunogen
This antibody was originally raised by immunizing BALB/c mice with ovarian carcinoma cell line OTN11.
Application Notes
Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometry. Cytokeratin 7 (CK7) labeling can help distinguish between lung, breast carcinomas, and urothelial carcinomas that typically stain positive, and colon and prostate carcinomas that typically lack CK7 expression (Jerome et al., 2004; Logani et al., 2003; Murray et al., 2004; Roy et al., 2011). CK7 is a common marker of primary lung adenocarcinomas (almost all cases) with a lower specificity since it is also observed in other primary lung carcinomas and non-pulmonary carcinomas (Jerome et al., 2004). Anti-cytokeratin 7 has also been useful in the differential diagnosis of ovarian neoplasms (McCluggage et al., 2005).
Antibody First Published
Ramaekers et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol. 1990 Mar; 136(3): 641–655. PMID:1690512
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
