Cat. No.
MABL-3917
Application
IP, radioimmunotherapy, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
TS1
From
Recombinant Antibody
Specificity
This antibody binds a discontinuous epitope on helical 2B domain of the human cytokeratin 8 between amino acid residues 340–365 (QRGELAIKDANAKLSELEAALQRAKQ). Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Together with KRT19, cytokeratin 8 helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Cytokeratin 8 is a serological tumor marker
Alternative Names
CK8; CK-8; CK 8; KRT8; CYK8; Keratin type II cytoskeletal 8; Cytokeratin- 8; Type-II keratin Kb8; TS 1; Mab 202
UniProt
P05787
Immunogen
This antibody was raised by immunising BALB/c mice with a synthetic peptide, known as 79E (LEEKKGNYVVTDHC), corresponding to the junctional sequence of the Δ2-7 EGFR deletion mutation.
Application Notes
This antibody has been used in fluorescence-activated cell sorting, Western blot and immunohistochemical analysis, using acetone-fixed frozen sections, of a tumour panel (Jungbluth et al, 2003). It does not stain normal tissues, which express wild-type EGFR (Jungbluth et al, 2003), and preferentially stains tumours expressing mutant receptors (Hills et al, 1995). This antibody has also been used in immunoprecipitation experiments (Hills et al, 1995) and ELISA analysis (Johns et al, 2002). This antibody has been shown to induce rapid internalisation of truncated EGFR by target cells (Johns et al, 2002), which could have therapeutic applications, given the tumour-restricted expression of ΔEGFR and its role in tumorigenesis.
Antibody First Published
Hills et al. Specific targeting of a mutant, activated FGF receptor found in glioblastoma using a monoclonal antibody. Int J Cancer. 1995 Nov 15;63(4):537-43. PMID:7591264
Note on publication
Describes the original generation of this antibody, and characterisation of its specificity in IP and WB experiments.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
