Rabbit anti-5-HT (Serotonin) 2C Receptor Antibody

Name

Rabbit anti-5-HT (Serotonin) 2C Receptor Antibody

中文名字

兔抗-5-HT 2C受体抗体，兔抗血清素抗体

Description

The ImmunoStar 5-HT2C receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat choroid plexus and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500 – 1/1000 in PBS – Bn/Av-HRP technique. Intensification methods such as nickel will approximately double the dilution factor as recommended.
The antibody was characterized by immunohistochemistry and Western blot. Western blotting revealed a single band of approximately 70 kD.  Due to the difficulty with receptor antibodies, western blot applications are not warranted and are included as specificity information only.
Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT2C receptors.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid

Reacts With

Mouse, Rat

Preabsorption Control

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Alternate Names

5-hydroxytryptamine receptor 2C; 5-HT2C; 5HT-1C; 5-HTR2C; 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled, anti-5-HT 2C

RRID

AB_572212

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Htr2c

Entrez Gene ID

Entrez Gene: 15560  Mouse
Entrez Gene: 25187  Rat

NCBI Gene Aliases

Sequence

>sp|P28335|5HT2C_HUMAN 5-hydroxytryptamine receptor 2C

APPLICATION

Quality Control

The 5-HT2C receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat choroid plexus and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Intensification methods such as nickel will approximately double the dilution factor as recommended. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with northern analysis, in situ hybridization and receptor autoradiography. The antibody was characterized by immunohistochemistry and western blot. Western blotting revealed a single band of approximately 70 kD. Due to the difficulty with receptor antibodies, western blot applications are not warranted and are included as specificity information only.

Tissue

Rat cortex, hippocampus, hypothalamic nuclei

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4.
• Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

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Sections

50 µm vibratome

Tissue Incubation

48 hours at 2°–8° C.

Detection System

Use Bn/AV-HRP at dilutions recommended by the manufacturer

Suggested Dilution

1/300–1/600 in PBS - Bn/Av-HRP technique
Note: Use of Triton X-100 or other detergents is not recommended.

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

Store at 2°–8°C until expiration date.