Cat. No | MBA-0056 |
Name | Rabbit anti-VIP (Vasoactive Intestinal Peptide) Antibody |
中文名字 | 兔抗VIP（血管活性肠肽）抗体；兔抗VIP抗体；兔抗血管活性肠肽抗体 |
Description | The antibody has a proven strong Biotin-Streptavidin/HRP staining at a 1/8000-1/10,000 dilution in rat amygdala, cortex, and suprachiasmatic nucleus. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Quantity/Volume | 50ul/100 µL |
State | Liquid Whole Serum/antibody |
Reacts With | Avian (Bird), Bat, Bubalus Bubalis (Buffalo), Bullfrog, Canine, Cat, Chick, Chicken, Dog, Dove, Fish, Fowl, Fox, Frog, Gilt (Pig), Goldfish, Guinea Pig, Hamster, Hedgehog, Hen, Human, Lizard, Mink, Mole, Mollusk, Monkey, Mouse, Mudpuppy (Necturus Maculosus), Ovine, Pig, Pigeon, Platypus, Possum, Rabbit, Raccoon, Ram, Rat, Scorpaena Porcus (Fish), Sea Lamprey, Sheep, Shrew, Snake, Sparrow, Sparus Aurata (Fish), Squirrel, Starfish, Sting Ray, Suncus Murinus, Tadpole, Turkey, Turtle, Worm (Spionidae), Zebra Finch, Zebrafish |
Preabsorption Control | Available at Sigma Aldrich, Catalog # V6130 |
Alternate Names | Vasoactive intestinal polypeptide; VIP peptides, anti-VIP |
RRID | AB_572270 |
Immunogen | This information is proprietary to Mabioway |
Gene Symbol | VIP |
Entrez Gene ID | Entrez Gene: 102254866 Bat |
NCBI Gene Aliases | / |
Sequence | P01282 |
APPLICATION | |
Quality Control | The antibody has significant Biotin/avidin-HRP staining at a 1/5000-1/10000 dilution in rat amygdala, cortex, and suprachiasmatic nucleus. The specificity of the antiserum was examined by soluble preadsorption with the peptides in question at a final concentration of 10-5 M. VIP immunolabeling was completely abolished by preadsorption with VIP. Preadsorption with the following peptides resulted in no reduction of immunostaining: secretin, gastric inhibitory polypeptide, somatostatin, glucagon, insulin, ACTH, gastrin 34, FMRF-amide, rat GHRF, human GHRF, peptide histidine isoleucine 27, rat pancreatic polypeptide, motilin, peptide YY, substance P, neuropeptide Y, and CGRP. |
Tissue | Rat amygdala, cortex and suprachiasmatic nucleus |
Perfusion Fixation | • Fixative: 4% paraformaldehyde in 0.1M Phosphate buffer, pH 7.4; 500 mL over 20 min. |
Absorption Control | |
Sections | 10 µm cryostat |
Tissue Incubation | 18–24 hours at 2°–8°C |
Detection System | Use Bn-AV/HRP or IF reagents at dilutions recommended by the manufacturers. |
Suggested Dilution | 1/5,000–1/10,000 in PBS/0.3% Triton X-100 – Bn-AV/HRP immunohistochemistry |
NOTES | |
Special Instructions | It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested. |
Concentration | Not applicable. Antibody concentration is only relevant for purified antibodies. |
Storage | After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles |
